Experiment AAV Tropism project Experiment Condition AAV8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000001959 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 75.32  1 77  2 72.77  3 93.91  4 57.62  Measurement Units: GC/ug DNA Mean 1010595  1 4421  2 282843  3 77426  4 167660  5 4780652  6 750568

AAV8 diaphragm AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging