Experiment AAV Tropism project Experiment Condition AAV8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000001965 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 99.45  1 99.91  2 98.98  3 99.22  4 99.68  Measurement Units: GC/ug DNA Mean 30861550.83  1 13722264  2 41199854  3 10002061  4 13697617  5 41175404  6 65372105

AAV8 liver AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging