Experiment AAV Tropism project Experiment Condition AAV8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: skin of body Measurement Type: Editing Efficiency Record ID 15000001969 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 25.92  1 20.49  2 12.74  3 42.3  4 28.16  Measurement Units: GC/ug DNA Mean 373820.2  1 179067  2 158778  3 181355  5 358132  6 991769

AAV8 skin of body AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging