Experiment AAV Tropism project Experiment Condition AAV9 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: gastrocnemius Measurement Type: Editing Efficiency Record ID 15000001981 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 76.41  1 84.56  2 94.17  3 64.54  4 62.36  Measurement Units: GC/ug DNA Mean 280304.5  1 184294  3 325243  4 532302  5 79379

AAV9 gastrocnemius AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging