Experiment AAV Tropism project Experiment Condition AAV9 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: large intestine Measurement Type: Editing Efficiency Record ID 15000001984 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 13.19  1 11.65  2 14.25  3 5.15  4 21.72  Measurement Units: GC/ug DNA Mean 10655.8  1 9801  2 23787  3 8034  4 8508  5 3149

AAV9 large intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging