Experiment AAV Tropism project Experiment Condition AAV9 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000001991 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 18.17  1 15.02  2 27.45  3 20.55  4 9.65  Measurement Units: GC/ug DNA Mean 18207  1 8499  2 12349  3 9342  4 9721  5 51124

AAV9 spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging