Experiment AAV Tropism project Experiment Condition AAV9 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: testis Measurement Type: Editing Efficiency Record ID 15000001994 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.79  1 5.58  2 4.16  3 5.43  4 12  Measurement Units: GC/ug DNA Mean 12085  1 14090  2 12588  3 8585  4 16677  5 8485

AAV9 testis AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging