Experiment AAV Tropism project Experiment Condition AAV9 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: eye Measurement Type: Editing Efficiency Record ID 15000002000 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 12.7  1 13.36  2 7.31  3 18.61  4 11.52  Measurement Units: GC/ug DNA Mean 10035.17  1 14791  2 2958  3 590  4 19279  5 15383  6 7210

AAV9 eye AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging