Experiment AAV Tropism project Experiment Condition AAV9 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: pancreas Measurement Type: Editing Efficiency Record ID 15000002008 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 31.97  1 44.02  2 28.03  3 33.29  4 22.54  Measurement Units: GC/ug DNA Mean 21350.5  1 102580  2 3113  3 10742  4 8015  5 1478  6 2175

AAV9 pancreas AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging