Experiment AAV Tropism project Experiment Condition AAV9 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: thymus Measurement Type: Editing Efficiency Record ID 15000002013 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 7.65  1 8.17  2 11.48  3 5.3  4 5.66  Measurement Units: GC/ug DNA Mean 9568  1 39716  2 23  3 16438  4 415  5 579  6 237

AAV9 thymus AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging