Experiment AAV Tropism project Experiment Condition AAV9 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: uterus Measurement Type: Editing Efficiency Record ID 15000002015 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 26.93  1 52.44  2 28.49  3 6.99  4 19.8  Measurement Units: GC/ug DNA Mean 6274.83  1 2765  2 1241  3 23921  4 8642  5 795  6 285

AAV9 uterus AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging