Experiment AAV Tropism project Experiment Condition AAVrh8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000002018 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 9.12  1 3.95  2 10.39  3 7.85  4 14.3  Measurement Units: GC/ug DNA Mean 21090.17  1 13672  2 11318  3 78857  4 15585  5 1137  6 5972

AAVrh8 brain AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging