Experiment AAV Tropism project Experiment Condition AAVrh8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000002022 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 96.09  1 97.1  2 93.66  3 95.33  4 98.27  Measurement Units: GC/ug DNA Mean 461804.5  1 317898  2 455716  3 457516  4 1064537  5 198667  6 276493

AAVrh8 heart AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging