Experiment AAV Tropism project Experiment Condition AAVrh8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: lymph node Measurement Type: Editing Efficiency Record ID 15000002027 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 12.42  1 11.68  2 19.69  3 9.38  4 8.92  Measurement Units: GC/ug DNA Mean 77899.5  1 41203  2 240885  3 56902  4 80668  5 33087  6 14652

AAVrh8 lymph node AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging