Experiment AAV Tropism project Experiment Condition AAVrh8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: small intestine Measurement Type: Editing Efficiency Record ID 15000002030 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 27.05  1 27.03  2 37.36  3 26.96  4 16.83  Measurement Units: GC/ug DNA Mean 17812.5  1 9103  2 34434  3 31470  4 23964  5 2727  6 5177

AAVrh8 small intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging