Experiment AAV Tropism project Experiment Condition AAVrh8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: epididymis Measurement Type: Editing Efficiency Record ID 15000002035 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 18.28  1 21.05  2 17.17  3 23.54  4 11.36  Measurement Units: GC/ug DNA Mean 20180.83  1 17894  2 19550  3 6997  4 71000  5 2905  6 2739

AAVrh8 epididymis AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging