Experiment AAV Tropism project Experiment Condition AAVrh8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000002045 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 99.21  1 99.52  2 99.23  3 99.68  4 98.4  Measurement Units: GC/ug DNA Mean 18754525.33  1 11084393  2 14222946  3 9979548  4 14536502  5 28343067  6 34360696

AAVrh8 liver AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging