Experiment AAV Tropism project Experiment Condition AAVrh8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000002051 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 21.09  1 25.62  2 21.37  3 23.03  4 14.33  Measurement Units: GC/ug DNA Mean 8654.5  1 5576  2 4928  3 5241  4 21504  5 7434  6 7244

AAVrh8 spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging