Experiment AAV Tropism project Experiment Condition AAVrh8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: ovary Measurement Type: Editing Efficiency Record ID 15000002054 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 36.27  1 36.89  2 43.33  3 40.34  4 24.51  Measurement Units: GC/ug DNA Mean 2842.4  1 934  2 1303  4 4736  5 2925  6 4314

AAVrh8 ovary AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging