Experiment AAV Tropism project Experiment Condition AAVrh10 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: gastrocnemius Measurement Type: Editing Efficiency Record ID 15000002061 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh10-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 92.77  1 99.86  2 92.57  3 87.84  4 90.8  Measurement Units: GC/ug DNA Mean 888919  1 627325  2 230198  3 1871652  4 730689  5 996941  6 876709

AAVrh10 gastrocnemius AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging