Experiment AAV Tropism project Experiment Condition AAVrh10 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000002062 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh10-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 98.76  1 98.83  2 99.23  3 98.17  4 98.8  Measurement Units: GC/ug DNA Mean 1569452.5  1 3825927  2 287165  3 1839078  4 1352048  5 947497  6 1165000

AAVrh10 heart AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging