Experiment AAV Tropism project Experiment Condition AAVrh10 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000002065 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh10-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 99.25  1 99.2  2 99.87  3 98.61  4 99.3  Measurement Units: GC/ug DNA Mean 44529062.33  1 26313547  2 37156203  3 44748312  4 32395078  5 73962934  6 52598300

AAVrh10 liver AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging