Experiment AAV Tropism project Experiment Condition AAVrh10 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000002079 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh10-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 81.06  1 74.18  2 75.33  3 81.17  4 93.57  Measurement Units: GC/ug DNA Mean 184285.33  1 428982  2 139634  3 70071  4 57895  5 263707  6 145423

AAVrh10 diaphragm AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging