Experiment AAV Tropism project Experiment Condition AAVrh10 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000002091 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh10-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 26.64  1 29.61  2 24.82  3 28.99  4 23.14  Measurement Units: GC/ug DNA Mean 26438.83  1 82587  2 23094  3 7984  4 4122  5 13596  6 27250

AAVrh10 spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging