Experiment AAV Tropism project Experiment Condition AAVrh74 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: urinary bladder Measurement Type: Editing Efficiency Record ID 15000002097 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 44.53  1 40.49  2 61.51  3 49.57  4 26.55  Measurement Units: GC/ug DNA Mean 25985.33  1 39801  2 58737  3 17806  4 16791  5 14466  6 8311

AAVrh74 urinary bladder AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging