Experiment AAV Tropism project Experiment Condition AAVrh74 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000002098 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 15.12  1 18.23  2 16.92  3 16.26  4 9.08  Measurement Units: GC/ug DNA Mean 8839.83  1 5029  2 13701  3 4735  4 14867  5 6679  6 8028

AAVrh74 brain AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging