Experiment AAV Tropism project Experiment Condition AAVrh74 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: lymph node Measurement Type: Editing Efficiency Record ID 15000002107 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 11.96  1 6.56  2 20.95  3 8.29  4 12.04  Measurement Units: GC/ug DNA Mean 56269.67  1 18317  2 51072  3 14255  4 37322  5 95489  6 121163

AAVrh74 lymph node AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging