Experiment AAV Tropism project Experiment Condition AAVrh74 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: skin of body Measurement Type: Editing Efficiency Record ID 15000002109 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 45.17  1 32.43  2 43.47  3 53.4  4 51.36  Measurement Units: GC/ug DNA Mean 127833.8  1 254296  2 160898  3 28589  4 116124  6 79262

AAVrh74 skin of body AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging