Experiment AAV Tropism project Experiment Condition AAVrh74 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: thymus Measurement Type: Editing Efficiency Record ID 15000002113 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 26.45  1 11.18  2 48.39  3 28.38  4 17.83  Measurement Units: GC/ug DNA Mean 1156.5  1 518  2 1991  3 195  4 1956  5 1258  6 1021

AAVrh74 thymus AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging