Experiment AAV Tropism project Experiment Condition AAVrh74 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000002118 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 17.98  1 17.68  2 24.36  3 12.22  4 17.66  Measurement Units: GC/ug DNA Mean 98082  1 17904  2 90470  3 43225  4 150572  5 267215  6 19106

AAVrh74 brain AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging