Experiment AAV Tropism project Experiment Condition AAVrh74 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000002125 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 99.25  1 99.11  2 99.47  3 99.19  4 99.23  Measurement Units: GC/ug DNA Mean 36429703.67  1 24422315  2 35227334  3 40243368  4 39959321  5 46233727  6 32492157

AAVrh74 liver AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging