Experiment AAV Tropism project Experiment Condition AAVrh74 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: lung Measurement Type: Editing Efficiency Record ID 15000002126 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 55.65  1 64.86  2 89.34  3 25.45  4 42.93  Measurement Units: GC/ug DNA Mean 546888.83  1 50672  2 605653  3 552772  4 617402  5 627821  6 827013

AAVrh74 lung AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging