Experiment AAV Tropism project Experiment Condition AAVrh74 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: lymph node Measurement Type: Editing Efficiency Record ID 15000002127 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAVrh74-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 10.83  1 12.59  3 13.49  4 6.4  Measurement Units: GC/ug DNA Mean 153244.83  1 135964  2 241210  3 66833  4 38579  5 280198  6 156685

AAVrh74 lymph node AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging