| AAV tropism in kidney organoids derived from human pluripotent stem cells. |
| AAV2/8 |
| Transduction efficiency was calculated by counting fluorescent cells (GFP) among LTL+ Proximal Tubule, CDH1+ Loop of Henle/Distal Tubule, PDGFR-B+ Interstitial, CD31+ Vascular, and PODXL+ podocyte cells. |
|
| Delivery Efficiency |
| 15000003401 |
|
|
| AAV |
| Viral vector |
|
| AAV2/8CMVeGFP; |
|
| Human |
| Human kidney organoid (stem cell-derived) |
|
| 33-44 |
|
| virus |
|
| MOI=10^5 |
|
|
|
| 7 days |
| AAV vector encoded |
|
|
|
| RRID:AB_300798 |
| ab13970 (Abcam) |
| Chicken anti-GFP polyclonal antibody |
|
| RRID:AB_2336558 |
| B-1325-2 (Vector Labs) |
| Lotus tetragonolobus lectin (LTL) |
|
| RRID:AB_298118 |
| ab11512 (Abcam) |
| Rat anti-e-cadherin monoclonal antibody |
|
| RRID:AB_777165 |
| ab32570 (Abcam) |
| Rabbit anti-PDGFRb monoclonal antibody |
|
| RRID:AB_307284 |
| ab9498 (Abcam) |
| Mouse anti-CD31 monoclonal antibody |
|
| RRID:AB_354920 |
| AF1658 (R&D Systems) |
| Goat anti-podocalyxin polyclonal antibody |
|
| Measured Values |
| Samples Size: | 6 |
| |
| Mean |
1.28 |
| 1 |
7.69 |
| 2 |
0 |
| 3 |
0 |
| 4 |
0 |
| 5 |
0 |
| 6 |
0 |
| Mean |
1.72 |
| 1 |
4.444 |
| 2 |
3.17 |
| 3 |
0 |
| 4 |
1.7 |
| 5 |
0 |
| 6 |
0.99 |
| Mean |
1.55 |
| 1 |
0 |
| 2 |
0.68 |
| 3 |
0.74 |
| 4 |
2.07 |
| 5 |
0.99 |
| 6 |
4.8 |
| Mean |
2.58 |
| 1 |
7.14 |
| 2 |
2.72 |
| 3 |
3.17 |
| 4 |
1.4 |
| 5 |
0 |
| 6 |
1.02 |
| Mean |
0.28 |
| 1 |
0 |
| 2 |
0 |
| 3 |
0 |
| 4 |
0 |
| 5 |
0.65 |
| 6 |
1.05 |