| AAV tropism in kidney organoids derived from human pluripotent stem cells. |
| AAV2/9 |
| Transduction efficiency was calculated by counting fluorescent cells (GFP) among LTL+ Proximal Tubule, CDH1+ Loop of Henle/Distal Tubule, PDGFR-B+ Interstitial, CD31+ Vascular, and PODXL+ podocyte cells. |
|
| Delivery Efficiency |
| 15000003402 |
|
|
| AAV |
| Viral vector |
|
| AAV2/9CMVeGFP; |
|
| Human |
| Human kidney organoid (stem cell-derived) |
|
| 33-44 |
|
| virus |
|
| MOI=10^5 |
|
|
|
| 7 days |
| AAV vector encoded |
|
|
|
| RRID:AB_300798 |
| ab13970 (Abcam) |
| Chicken anti-GFP polyclonal antibody |
|
| RRID:AB_2336558 |
| B-1325-2 (Vector Labs) |
| Lotus tetragonolobus lectin (LTL) |
|
| RRID:AB_298118 |
| ab11512 (Abcam) |
| Rat anti-e-cadherin monoclonal antibody |
|
| RRID:AB_777165 |
| ab32570 (Abcam) |
| Rabbit anti-PDGFRb monoclonal antibody |
|
| RRID:AB_307284 |
| ab9498 (Abcam) |
| Mouse anti-CD31 monoclonal antibody |
|
| RRID:AB_354920 |
| AF1658 (R&D Systems) |
| Goat anti-podocalyxin polyclonal antibody |
|
| Measured Values |
| Samples Size: | 6 |
| |
| Mean |
0 |
| 1 |
0 |
| 2 |
0 |
| 3 |
0 |
| 4 |
0 |
| 5 |
0 |
| 6 |
0 |
| Mean |
1.47 |
| 1 |
0 |
| 2 |
4.5 |
| 3 |
0 |
| 4 |
0.71 |
| 5 |
2.49 |
| 6 |
1.14 |
| Mean |
4.73 |
| 1 |
4.3 |
| 2 |
2 |
| 3 |
4 |
| 4 |
4.16 |
| 5 |
5.62 |
| 6 |
8.28 |
| Mean |
1.84 |
| 1 |
4.17 |
| 2 |
2.67 |
| 3 |
0 |
| 4 |
2.53 |
| 5 |
1.68 |
| 6 |
0 |
| Mean |
1.47 |
| 1 |
0 |
| 2 |
4.5 |
| 3 |
0 |
| 4 |
0.71 |
| 5 |
2.49 |
| 6 |
1.14 |