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Delivery Systems
 AAV
Models
 Human kidney organoid (iPSC-derived)
Sex
 Male
Vectors
 AAV2/2CMVeGFP
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Results

AAV2/2_transduction_efficiencies_in_kidney_organoids_cultured_under_flow_determined_by_FACS
Delivery Efficiency
Record Id Condition Sex Model Delivery Vector Dosage Delivery Efficiency %GFP+ / total cells Delivery Efficiency % GFP+ LTL+ / LTL+ cells Image
15000003419 High flow (AAV-) Male Human kidney organoid (iPSC-derived) control
15000003420 High flow Male Human kidney organoid (iPSC-derived) AAV AAV2/2CMVeGFP MOI=10^5
15000003421 Pulsed flow Male Human kidney organoid (iPSC-derived) AAV AAV2/2CMVeGFP MOI=10^5
15000003422 No flow Male Human kidney organoid (iPSC-derived) AAV AAV2/2CMVeGFP MOI=10^5
15000003423 U-well Male Human kidney organoid (iPSC-derived) AAV AAV2/2CMVeGFP MOI=10^5

AAV2/2 transduction efficiencies in kidney organoids cultured under flow (A) Flow cytometry of kidney organoids exposed to AAV2/2 at MOI 10^5 for 1 day in four culture conditions. (B) Graphs showing percentages of transduced cells by AAV2/2 in LTL+ proximal tubules.