Experiment Podocyte-specific gene editing in human kidney organoids Experiment Condition RNP Assay Description: Calculated Editing efficiency by counting fluorescent cells (TdTomato) compared to Wilms Tumor-1 (WT1) stained cells. NOTE: Each data point represents one organoid. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003587 Editor SpyCas9-ProteinG-3xNLS Delivery System endosomolytic peptide Delivery System Subtype Amphiphilic peptide Guide sg298 Vector Model Species human Model Name human kidney organoid with Ai9 (tdTomato) fluorescence-on reporter Application Method co-incubation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Antibody RRID RRID:AB_2043201 Antibody Other ID Abcam (ab89901) Antibody Description anti-Wilms Tumor-1 (WT1) Measured Values Samples Size: 17   Replicate Result (Editing Efficiency) Measurement Units: WT1+tdTomato+/total tdTomato+ (%) Mean 14.9  1 0  2 0  3 0  4 25  5 0  6 25  7 16.6666667  8 0  9 0  10 0  11 20  12 0  13 0  14 28.5714286  15 0  16 66.6666667  17 71.4285714