Experiment Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Heterozygous Blastocysts using SaCas9 Experiment Condition SaC9-g3 Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000005236 Editor sNLS-SaCas9-sNLS Nuclease Delivery System Delivery System Subtype Guide SaC9-g3 Vector Model Species Mouse Model Name TLR-2 Heterozygous Blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: # blasts assessed by sequencing Mean # blasts analyzed  1 55 blasts  Measurement Units: # blasts assessed for RFP activation Mean # blasts analyzed  1 55 blasts  Measurement Units: % of blasts with RFP activation Mean 13  1 13.0  Measurement Units: % of blasts with edits Mean 74  1 74.0