Experiment: Analysis of editing outcomes and adverse events in human kidney organoids
PI: Benjamin S Freedman, PhD
Description: Kidney organoids were derived from a human iPS cell line expressing GFP from the safe harbor locus. These were transfected with a commercially available CRISPR ribonucleoprotein complex with guide RNA targeting GFP or a scrambled sequence (control). Editing events were assessed by next generation sequencing of the GFP locus.
Editing Assay:
Calculated editing efficiency by quantifying indels as a percentage of total reads by amplicon-based NGS. Each data point represents a distinct biological replicate (independent experiment).
Download: Submitted files
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
| Editing Efficiency | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Condition | Cell Type | Sex | Editor | Model | Delivery | Target Locus | Guide | % of reads | Image |
| scr gRNA | Male | Alt-R™ S.p. HiFi Cas9 Nuclease V3 | kidney organoid | CRISPRMAX | Alt-R™ CRISPR-Cas9 Negative Control crRNA #1 | ||||
| GFP gRNA | Male | Alt-R™ S.p. HiFi Cas9 Nuclease V3 | kidney organoid | CRISPRMAX | AAVS1 | GFP-cRNA | |||
× Close Overview
