Experiment Genome editing activity of four Streptococcus Cas9s by gRNA targeting the HBE promoter in HEK293T cells. Experiment Condition SpaCas9-sgRNA2 Assay Description: Calculated editing efficiency by NGS sequencing at target site. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000005350 Editor S. parasanguinis Cas9 Delivery System Lipofectamine 3000 Delivery System Subtype Commercial Reagent Guide SpaCas9-sgRNA2 Vector Model Species Human Model Name HEK-293T Application Method Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 3.11  1 2.98551769470537  2 3.25520833333333  3 3.09759283448405