AAVS1_site_01 | SCGE Toolkit

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SCGE ID	10000000089
Lab ID	AAVS1_site_01
Source	lab IVT
Target Locus	AAVS1
Species	human
Description	Targets AAVS1 safe harbor locus
Guide Compatibility	SpyCas9

Target Sequence	GTCACCAATCCTGTCCCTAG
Target Sequence + PAM	GTCACCAATCCTGTCCCTAGNGG
Position	hg38/chr19:55115744-55115767

Spacer Sequence	GUCACCAAUCCUGUCCCUAG
Spacer Length	20
Modifications	none

Forward Primer	CTGCCTAACAGGAGGTGGGGGTT
Reverse Primer	ACCCGGGCCCCTATGTCCACTTC

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	275
Guide-seq	7

Specificity Ratio	0.02

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: AAVS1_site_01 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)