A novel human T cell platform to define biological adverse effects of genome editing

Experiment: A novel human T cell platform to define biological adverse effects of genome editing

PI: Shengdar Q Tsai, PhD>

Description: 110 guide RNAs and SpCas9 were transfected into human T-cells. Indel rates were measured by targeted amplicon deep sequencing.

Editing Assay: Calculated editing efficiency by targeted amplicon deep sequencing

Parent Project: A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing

Download: Submitted files

Publications:

CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

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Results

A_novel_human_T_cell_platform_to_define_biological_adverse_effects_of_genome_editing
								Editing Efficiency
Record Id	Condition	Sex	Editor	Model	Delivery	Target Locus	Guide	Dosage	Editing Efficiency % of reads	Image
15000000421	AAVS1_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	31.5704111134617; 29.5114085058926; 27.6135496499784;
15000000422	AAVS1_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	70.8102374597961; 70.119329648153; 66.5487784971979;
15000000423	AAVS1_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	37.4685696072529; 38.0383917486391; 36.6466084874453;
15000000424	AAVS1_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	32.7489009568141; 34.7192745514181; 33.8946517580493;
15000000425	AAVS1_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	31.755192126782; 31.5499245060067; 33.2177634743949;
15000000426	AAVS1_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	8.87801378462169; 7.54347884342257; 5.69997164193213;
15000000427	AAVS1_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	20.2621246474589; 21.3278090164743; 21.0225323115635;
15000000428	AAVS1_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	71.2144225054481; 63.1950399050195; 65.5152394775036;
15000000429	AAVS1_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	36.9420010348558; 36.3026124818578; 37.2728180547234;
15000000430	AAVS1_site_10	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_10	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	89.65650328058659; 89.7069167643611; 89.990964762574;
15000000431	AAVS1_site_11	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_11	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	93.01537836189961; 93.6252472576875; 92.3916913946588;
15000000432	AAVS1_site_12	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_12	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.8380112269447; 88.043065231159; 89.5858508184115;
15000000433	AAVS1_site_13	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_13	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	41.698918150234896; 41.7140358548317; 40.1194747062502;
15000000434	AAVS1_site_14	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	AAVS1	AAVS1_site_14	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	89.0502746795405; 87.23848532305341; 88.0111397907893;
15000000435	B2M_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.8509271158436; 96.4009140535737; 95.8541128402331;
15000000436	B2M_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	32.233777488896095; 31.269204728920403; 31.8891952212157;
15000000437	B2M_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	96.2598122210251; 96.32393895510751; 96.2989360077381;
15000000438	B2M_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.6380583337697; 94.9095655416744; 95.0261308343846;
15000000439	B2M_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	97.4216335540839; 96.9031531531532; 97.3593662478995;
15000000440	B2M_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	77.0133793698748; 77.77684422786089; 76.1116481391977;
15000000441	B2M_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	B2M	B2M_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.8672086720867; 93.9055717955532; 95.3293665420462;
15000000442	CBLB_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CBLB	CBLB_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	69.2378328741965; 69.5883455237865; 68.777448293983;
15000000443	CBLB_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CBLB	CBLB_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	77.22527829514449; 81.8546668560875; 81.668376706153;
15000000444	CBLB_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CBLB	CBLB_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	25.4096432771462; 26.791209977863; 24.5504562533548;
15000000445	CBLB_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CBLB	CBLB_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	76.7408647690338; 74.7433689286945; 75.0096505033109;
15000000446	CBLB_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CBLB	CBLB_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	63.639277015767206; 61.957490572506; 63.990366479993;
15000000447	CCR5_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	74.27094762372231; 74.5206705449038; 74.3426114505544;
15000000448	CCR5_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	23.0266502069566; 20.4204309769044; 21.779552343203502;
15000000449	CCR5_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	42.370624048706205; 42.407689116304795; 43.1019949423996;
15000000450	CCR5_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	87.4945273631841; 86.7245820055005; 88.2399577167019;
15000000451	CCR5_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	91.1168237646387; 90.4720234724867; 91.2759560274359;
15000000452	CCR5_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	46.0191568543713; 45.9768095572734; 45.8696386355961;
15000000453	CCR5_site_11	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_11	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	82.6646090534979; 79.6880154578444; 84.1311509162034;
15000000454	CCR5_site_13	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_13	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	32.5465134257589; 30.9114183307732; 33.715870771239;
15000000455	CCR5_site_14	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_14	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	50.14624246403629; 71.289818092024; 70.76681496990321;
15000000456	CCR5_site_15	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_15	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	56.11985341668459; 56.1475409836066; 58.3479141725757;
15000000457	CCR5_site_16	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CCR5	CCR5_site_16	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	25.8704557091654; 24.2438408676546; 24.6824131768343;
15000000458	CTLA4_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.0864260294865; 88.506711409396; 87.7025738798856;
15000000459	CTLA4_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	52.25092250922509; 52.741103985917306; 53.9530426449449;
15000000460	CTLA4_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	30.902920608802997; 29.2738240125563; 30.165596964646603;
15000000461	CTLA4_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	94.6651155060661; 94.0878558481714; 94.3203371970495;
15000000462	CTLA4_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	23.9051012584006; 20.8143980379427; 22.4767231918434;
15000000463	CTLA4_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	91.6124186279419; 90.8297843110317; 91.0814170292107;
15000000464	CTLA4_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	14.8821922881081; 14.287136103508299; 15.4795547609435;
15000000465	CTLA4_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	92.3076923076923; 92.94227494919541; 93.2322181459782;
15000000466	CTLA4_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	82.3347107438017; 83.9546880816891; 82.1307182258873;
15000000467	CTLA4_site_10	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_10	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	76.0455634483628; 74.41511697660471; 77.3001139879498;
15000000468	CTLA4_site_11	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_11	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	48.6247831091412; 51.447259854675195; 51.048503281575194;
15000000469	CTLA4_site_12	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_12	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	78.7009385665529; 89.2809118268553; 89.62616231260671;
15000000470	CTLA4_site_13	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CTLA4	CTLA4_site_13	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	41.985221787568896; 47.9598982713501; 47.5282281412855;
15000000471	CXCR4_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	70.5947063137543; 71.8857832988268; 72.6924462198356;
15000000472	CXCR4_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	57.63905639442331; 59.1441419182685; 57.1028769730214;
15000000473	CXCR4_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	80.5432999088423; 79.07487642643561; 80.3266163690192;
15000000474	CXCR4_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	87.130778522016; 88.2853437094682; 86.42044920590169;
15000000475	CXCR4_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	83.6295508633349; 81.7379589087235; 84.226624956552;
15000000476	CXCR4_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.2427851580394; 92.8931480053417; 92.8219634783926;
15000000477	CXCR4_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.064162172215; 88.214831602804; 87.6661870503597;
15000000478	CXCR4_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	76.2026000077152; 76.6770303372904; 76.5135231954147;
15000000479	CXCR4_site_10	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_10	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	53.2908005762503; 56.099015806740205; 66.65235200845669;
15000000480	CXCR4_site_11	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	CXCR4	CXCR4_site_11	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	46.413995364958296; 44.4398011701051; 43.7219951745532;
15000000481	FAS_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	FAS	FAS_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.2443413042747; 94.7736436482417; 94.7674723431028;
15000000482	FAS_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	FAS	FAS_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	74.0212919370573; 73.7380462413751; 70.67068843151169;
15000000483	FAS_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	FAS	FAS_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	92.8114496367776; 92.82359317134859; 89.8120716338713;
15000000484	FAS_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	FAS	FAS_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	96.7659027629043; 96.1457857334195; 96.01363213879999;
15000000485	FAS_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	FAS	FAS_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	96.48243377554991; 95.4315465278287; 94.81251480802561;
15000000486	LAG3_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	59.5187580853816; 55.6302832109582; 56.804225006140996;
15000000487	LAG3_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	42.0421404463086; 38.1214137615121; 41.8073528151513;
15000000488	LAG3_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	67.9600783509001; 64.9848962015554; 63.745676165376395;
15000000489	LAG3_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	3.00917775743492; 2.9891041292446; 2.9279643868879;
15000000490	LAG3_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	20.0051604912788; 19.2077244708824; 19.7999141348083;
15000000491	LAG3_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	1.9767291986454698; 1.81470579309926; 1.8179784973720798;
15000000492	LAG3_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	15.448880233690401; 12.0802448759115; 15.208864856631498;
15000000493	LAG3_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	51.700598127530796; 52.758489720791204; 58.46772510331581;
15000000494	LAG3_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	64.4555754096491; 64.5146825488077; 58.870851730128706;
15000000495	LAG3_site_10	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	LAG3	LAG3_site_10	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	42.9803152617563; 43.130410638319; 43.7608102483467;
15000000496	PDCD1_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	89.060731799321; 2.39371649420272; 88.55592169025009;
15000000497	PDCD1_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	94.55063033753561; 94.1460126516644; 94.2970045858132;
15000000498	PDCD1_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.6048318493733; 94.9339207048458; 94.2241657617468;
15000000499	PDCD1_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	75.1895991332611; 75.40015147689981; 73.8051177333929;
15000000500	PDCD1_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	60.772478280590306; 58.8948528706987; 58.8316954663354;
15000000501	PDCD1_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	13.9203507489952; 10.8708654281831; 10.9403462851281;
15000000502	PDCD1_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	8.15373254795556; 7.236318124928889; 7.263829691270331;
15000000503	PDCD1_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	4.39565605754313; 3.61448244861264; 4.0434232102455505;
15000000504	PDCD1_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	40.953096881477904; 42.3341961074322; 44.3563172578717;
15000000505	PDCD1_site_10	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_10	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	6.597194706395119; 7.44081710576556; 7.37380786229356;
15000000506	PDCD1_site_11	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_11	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	47.504853670813304; 50.8485812781756; 51.3387198986058;
15000000507	PDCD1_site_12	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_12	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	7.93437002571324; 8.867856281649381; 8.037592930284749;
15000000508	PDCD1_site_13	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_13	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	48.6442766908027; 45.3677955683049; 48.318911923672296;
15000000509	PDCD1_site_14	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_14	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	12.6249828790577; 12.2567264255476; 12.6877064423904;
15000000510	PDCD1_site_15	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_15	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	83.38928909025721; 81.92674579480081; 81.4785472011092;
15000000511	PDCD1_site_16	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PDCD1	PDCD1_site_16	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	34.643321897551196; 39.4182568334193; 36.7016705443417;
15000000512	PTPN2_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN2	PTPN2_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	91.9523503333446; 89.4053808990834; 92.6307584082742;
15000000513	PTPN2_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN2	PTPN2_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.3917332395451; 92.9782932005414; 92.3171674926237;
15000000514	PTPN2_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN2	PTPN2_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	66.98215909527529; 66.52532459936839; 64.2576630795124;
15000000515	PTPN6_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	91.9531377029259; 92.1467796717773; 92.0578968711982;
15000000516	PTPN6_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.2361820581989; 86.53193804403479; 93.41094096886209;
15000000517	PTPN6_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	79.1606210098665; 78.1767701558473; 78.3928732385903;
15000000518	PTPN6_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	84.29744441369131; 85.4498396444044; 85.465310459997;
15000000519	PTPN6_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	32.1636850815667; 32.940456145721704; 32.036727592206596;
15000000520	PTPN6_site_06	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_06	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	95.511562865253; 96.155936058634; 96.16958033922201;
15000000521	PTPN6_site_07	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_07	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	96.6125386115377; 96.8411071927607; 97.3255225085887;
15000000522	PTPN6_site_08	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_08	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	60.314017696760004; 60.862543642432; 62.9770738140594;
15000000523	PTPN6_site_09	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	PTPN6	PTPN6_site_09	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	90.6155884833153; 90.50755662897501; 90.9079203618162;
15000000524	TRAC_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRAC	TRAC_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	87.0788126186128; 88.0390221682356; 86.9165679471992;
15000000525	TRAC_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRAC	TRAC_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	86.771397191496; 86.94808848352041; 88.0861550456672;
15000000526	TRAC_site_03	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRAC	TRAC_site_03	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	84.3907561460606; 84.519734400132; 85.12774605632441;
15000000527	TRAC_site_04	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRAC	TRAC_site_04	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	72.9512991301394; 69.3104294478528; 72.4644427204551;
15000000528	TRAC_site_05	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRAC	TRAC_site_05	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	1.2335557673975202; 1.10708237192967; 2.02669528779073;
15000000529	TRBC1_site_01	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRBC1	TRBC1_site_01	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	83.3198913320316; 86.8394939221037; 80.03155739080809;
15000000530	TRBC1_site_02	Male	SpCas9	CD4/CD8 Human Primary T cell	P3 Nucleofection Kit	TRBC1	TRBC1_site_02	75 pmol Cas9 protein; 3-fold molar excess of sgRNA	79.02064584436211; 78.01934123463971; 74.73605297682259;

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

Title	Description	File Download	SCGE ID
Tsai_T Cell Transfection Protocol	Procedure for T cell transfection.	Tsai_T_cell_transfection.pdf	21000000006

Experiment: A novel human T cell platform to define biological adverse effects of genome editing

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Experiment: A novel human T cell platform to define biological adverse effects of genome editing

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