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Experiment: A novel human T cell platform to define biological adverse effects of genome editing

PI:   Shengdar Q Tsai, PhD 

Description: 110 guide RNAs and SpCas9 were transfected into human T-cells. Indel rates were measured by targeted amplicon deep sequencing.
Editing Assay: Calculated editing efficiency by targeted amplicon deep sequencing
Download: Submitted files
Publications:  CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI
Filter Results ..
Editors
 SpCas9
  Guide Target Locus
 AAVS1
 B2M
 CBLB
 CCR5
 CTLA4
 CXCR4
 FAS
 LAG3
 PDCD1
 PTPN2
 PTPN6
 TRAC
 TRBC1
  Guides
 AAVS1_site_01
 AAVS1_site_02
 AAVS1_site_03
 AAVS1_site_04
 AAVS1_site_05
 AAVS1_site_06
 AAVS1_site_07
 AAVS1_site_08
 AAVS1_site_09
 AAVS1_site_10
 AAVS1_site_11
 AAVS1_site_12
 AAVS1_site_13
 AAVS1_site_14
 B2M_site_01
 B2M_site_02
 B2M_site_03
 B2M_site_04
 B2M_site_05
 B2M_site_06
 B2M_site_07
 CBLB_site_04
 CBLB_site_05
 CBLB_site_06
 CBLB_site_08
 CBLB_site_09
 CCR5_site_03
 CCR5_site_04
 CCR5_site_05
 CCR5_site_07
 CCR5_site_08
 CCR5_site_09
 CCR5_site_11
 CCR5_site_13
 CCR5_site_14
 CCR5_site_15
 CCR5_site_16
 CTLA4_site_01
 CTLA4_site_02
 CTLA4_site_03
 CTLA4_site_04
 CTLA4_site_05
 CTLA4_site_06
 CTLA4_site_07
 CTLA4_site_08
 CTLA4_site_09
 CTLA4_site_10
 CTLA4_site_11
 CTLA4_site_12
 CTLA4_site_13
 CXCR4_site_01
 CXCR4_site_02
 CXCR4_site_03
 CXCR4_site_04
 CXCR4_site_05
 CXCR4_site_06
 CXCR4_site_07
 CXCR4_site_08
 CXCR4_site_10
 CXCR4_site_11
 FAS_site_01
 FAS_site_02
 FAS_site_03
 FAS_site_04
 FAS_site_05
 LAG3_site_01
 LAG3_site_02
 LAG3_site_03
 LAG3_site_04
 LAG3_site_05
 LAG3_site_06
 LAG3_site_07
 LAG3_site_08
 LAG3_site_09
 LAG3_site_10
 PDCD1_site_01
 PDCD1_site_02
 PDCD1_site_03
 PDCD1_site_04
 PDCD1_site_05
 PDCD1_site_06
 PDCD1_site_07
 PDCD1_site_08
 PDCD1_site_09
 PDCD1_site_10
 PDCD1_site_11
 PDCD1_site_12
 PDCD1_site_13
 PDCD1_site_14
 PDCD1_site_15
 PDCD1_site_16
 PTPN2_site_01
 PTPN2_site_02
 PTPN2_site_03
 PTPN6_site_01
 PTPN6_site_02
 PTPN6_site_03
 PTPN6_site_04
 PTPN6_site_05
 PTPN6_site_06
 PTPN6_site_07
 PTPN6_site_08
 PTPN6_site_09
 TRAC_site_01
 TRAC_site_02
 TRAC_site_03
 TRAC_site_04
 TRAC_site_05
 TRBC1_site_01
 TRBC1_site_02
Delivery Systems
 P3 Nucleofection Kit
Models
 CD4/CD8 Human Primary T cell
Sex
 Male
Select experimental variable to highlight records on the chart:

Note: Hover over the bars to view additional information

Results
A_novel_human_T_cell_platform_to_define_biological_adverse_effects_of_genome_editing
Editing Efficiency
Record Id Condition Sex Editor Model Delivery Target Locus Guide Dosage Editing Efficiency % of reads Image
15000000421 AAVS1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000422 AAVS1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000423 AAVS1_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000424 AAVS1_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000425 AAVS1_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000426 AAVS1_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000427 AAVS1_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000428 AAVS1_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000429 AAVS1_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000430 AAVS1_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000431 AAVS1_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000432 AAVS1_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000433 AAVS1_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000434 AAVS1_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000435 B2M_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000436 B2M_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000437 B2M_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000438 B2M_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000439 B2M_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000440 B2M_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000441 B2M_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000442 CBLB_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000443 CBLB_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000444 CBLB_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000445 CBLB_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000446 CBLB_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000447 CCR5_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000448 CCR5_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000449 CCR5_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000450 CCR5_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000451 CCR5_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000452 CCR5_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000453 CCR5_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000454 CCR5_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000455 CCR5_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000456 CCR5_site_15 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_15 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000457 CCR5_site_16 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_16 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000458 CTLA4_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000459 CTLA4_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000460 CTLA4_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000461 CTLA4_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000462 CTLA4_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000463 CTLA4_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000464 CTLA4_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000465 CTLA4_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000466 CTLA4_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000467 CTLA4_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000468 CTLA4_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000469 CTLA4_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000470 CTLA4_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000471 CXCR4_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000472 CXCR4_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000473 CXCR4_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000474 CXCR4_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000475 CXCR4_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000476 CXCR4_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000477 CXCR4_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000478 CXCR4_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000479 CXCR4_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000480 CXCR4_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000481 FAS_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000482 FAS_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000483 FAS_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000484 FAS_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000485 FAS_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000486 LAG3_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000487 LAG3_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000488 LAG3_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000489 LAG3_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000490 LAG3_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000491 LAG3_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000492 LAG3_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000493 LAG3_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000494 LAG3_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000495 LAG3_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000496 PDCD1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000497 PDCD1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000498 PDCD1_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000499 PDCD1_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000500 PDCD1_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000501 PDCD1_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000502 PDCD1_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000503 PDCD1_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000504 PDCD1_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000505 PDCD1_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000506 PDCD1_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000507 PDCD1_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000508 PDCD1_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000509 PDCD1_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000510 PDCD1_site_15 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_15 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000511 PDCD1_site_16 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_16 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000512 PTPN2_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000513 PTPN2_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000514 PTPN2_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000515 PTPN6_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000516 PTPN6_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000517 PTPN6_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000518 PTPN6_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000519 PTPN6_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000520 PTPN6_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000521 PTPN6_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000522 PTPN6_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000523 PTPN6_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000524 TRAC_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000525 TRAC_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000526 TRAC_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000527 TRAC_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000528 TRAC_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000529 TRBC1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRBC1 TRBC1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000530 TRBC1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRBC1 TRBC1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA

Associated Publications
Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

Experiment Wide Protocols

Title Description File Download SCGE ID
Tsai_T Cell Transfection Protocol Procedure for T cell transfection. Tsai_T_cell_transfection.pdf 21000000006