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Experiment: A novel human T cell platform to define biological adverse effects of genome editing

PI:   Shengdar Q Tsai, PhD 
 
 
110 guide RNAs and SpCas9 were transfected into human T-cells. Indel rates were measured by targeted amplicon deep sequencing.
Editing Assay: - Calculated editing efficiency by targeted amplicon deep sequencing

Filter Results ..
Editors
 SpCas9
  Guide Target Locus
 AAVS1
 B2M
 CBLB
 CCR5
 CTLA4
 CXCR4
 FAS
 LAG3
 PDCD1
 PTPN2
 PTPN6
 TRAC
 TRBC1
  Guides
 AAVS1_site_01
 AAVS1_site_02
 AAVS1_site_03
 AAVS1_site_04
 AAVS1_site_05
 AAVS1_site_06
 AAVS1_site_07
 AAVS1_site_08
 AAVS1_site_09
 AAVS1_site_10
 AAVS1_site_11
 AAVS1_site_12
 AAVS1_site_13
 AAVS1_site_14
 B2M_site_01
 B2M_site_02
 B2M_site_03
 B2M_site_04
 B2M_site_05
 B2M_site_06
 B2M_site_07
 CBLB_site_04
 CBLB_site_05
 CBLB_site_06
 CBLB_site_08
 CBLB_site_09
 CCR5_site_03
 CCR5_site_04
 CCR5_site_05
 CCR5_site_07
 CCR5_site_08
 CCR5_site_09
 CCR5_site_11
 CCR5_site_13
 CCR5_site_14
 CCR5_site_15
 CCR5_site_16
 CTLA4_site_01
 CTLA4_site_02
 CTLA4_site_03
 CTLA4_site_04
 CTLA4_site_05
 CTLA4_site_06
 CTLA4_site_07
 CTLA4_site_08
 CTLA4_site_09
 CTLA4_site_10
 CTLA4_site_11
 CTLA4_site_12
 CTLA4_site_13
 CXCR4_site_01
 CXCR4_site_02
 CXCR4_site_03
 CXCR4_site_04
 CXCR4_site_05
 CXCR4_site_06
 CXCR4_site_07
 CXCR4_site_08
 CXCR4_site_10
 CXCR4_site_11
 FAS_site_01
 FAS_site_02
 FAS_site_03
 FAS_site_04
 FAS_site_05
 LAG3_site_01
 LAG3_site_02
 LAG3_site_03
 LAG3_site_04
 LAG3_site_05
 LAG3_site_06
 LAG3_site_07
 LAG3_site_08
 LAG3_site_09
 LAG3_site_10
 PDCD1_site_01
 PDCD1_site_02
 PDCD1_site_03
 PDCD1_site_04
 PDCD1_site_05
 PDCD1_site_06
 PDCD1_site_07
 PDCD1_site_08
 PDCD1_site_09
 PDCD1_site_10
 PDCD1_site_11
 PDCD1_site_12
 PDCD1_site_13
 PDCD1_site_14
 PDCD1_site_15
 PDCD1_site_16
 PTPN2_site_01
 PTPN2_site_02
 PTPN2_site_03
 PTPN6_site_01
 PTPN6_site_02
 PTPN6_site_03
 PTPN6_site_04
 PTPN6_site_05
 PTPN6_site_06
 PTPN6_site_07
 PTPN6_site_08
 PTPN6_site_09
 TRAC_site_01
 TRAC_site_02
 TRAC_site_03
 TRAC_site_04
 TRAC_site_05
 TRBC1_site_01
 TRBC1_site_02
Models
 CD4/CD8 Human Primary T cell
Sex
 Male
Make a selection to highlight records on the chart:

Results
A_novel_human_T_cell_platform_to_define_biological_adverse_effects_of_genome_editing
Editing Efficiency
Record Id Condition Sex Editor Model Delivery Target Locus Guide Dosage Editing Efficiency % of reads Image
15000000421 AAVS1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000422 AAVS1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000423 AAVS1_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000424 AAVS1_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000425 AAVS1_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000426 AAVS1_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000427 AAVS1_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000428 AAVS1_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000429 AAVS1_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000430 AAVS1_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000431 AAVS1_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000432 AAVS1_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000433 AAVS1_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000434 AAVS1_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit AAVS1 AAVS1_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000435 B2M_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000436 B2M_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000437 B2M_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000438 B2M_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000439 B2M_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000440 B2M_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000441 B2M_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit B2M B2M_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000442 CBLB_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000443 CBLB_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000444 CBLB_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000445 CBLB_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000446 CBLB_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CBLB CBLB_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000447 CCR5_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000448 CCR5_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000449 CCR5_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000450 CCR5_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000451 CCR5_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000452 CCR5_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000453 CCR5_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000454 CCR5_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000455 CCR5_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000456 CCR5_site_15 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_15 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000457 CCR5_site_16 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CCR5 CCR5_site_16 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000458 CTLA4_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000459 CTLA4_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000460 CTLA4_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000461 CTLA4_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000462 CTLA4_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000463 CTLA4_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000464 CTLA4_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000465 CTLA4_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000466 CTLA4_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000467 CTLA4_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000468 CTLA4_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000469 CTLA4_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000470 CTLA4_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CTLA4 CTLA4_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000471 CXCR4_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000472 CXCR4_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000473 CXCR4_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000474 CXCR4_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000475 CXCR4_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000476 CXCR4_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000477 CXCR4_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000478 CXCR4_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000479 CXCR4_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000480 CXCR4_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit CXCR4 CXCR4_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000481 FAS_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000482 FAS_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000483 FAS_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000484 FAS_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000485 FAS_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit FAS FAS_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000486 LAG3_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000487 LAG3_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000488 LAG3_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000489 LAG3_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000490 LAG3_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000491 LAG3_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000492 LAG3_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000493 LAG3_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000494 LAG3_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000495 LAG3_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit LAG3 LAG3_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000496 PDCD1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000497 PDCD1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000498 PDCD1_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000499 PDCD1_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000500 PDCD1_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000501 PDCD1_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000502 PDCD1_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000503 PDCD1_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000504 PDCD1_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000505 PDCD1_site_10 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_10 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000506 PDCD1_site_11 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_11 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000507 PDCD1_site_12 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_12 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000508 PDCD1_site_13 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_13 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000509 PDCD1_site_14 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_14 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000510 PDCD1_site_15 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_15 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000511 PDCD1_site_16 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PDCD1 PDCD1_site_16 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000512 PTPN2_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000513 PTPN2_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000514 PTPN2_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN2 PTPN2_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000515 PTPN6_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000516 PTPN6_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000517 PTPN6_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000518 PTPN6_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000519 PTPN6_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000520 PTPN6_site_06 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_06 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000521 PTPN6_site_07 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_07 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000522 PTPN6_site_08 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_08 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000523 PTPN6_site_09 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit PTPN6 PTPN6_site_09 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000524 TRAC_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000525 TRAC_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000526 TRAC_site_03 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_03 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000527 TRAC_site_04 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_04 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000528 TRAC_site_05 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRAC TRAC_site_05 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000529 TRBC1_site_01 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRBC1 TRBC1_site_01 75 pmol Cas9 protein; 3-fold molar excess of sgRNA
15000000530 TRBC1_site_02 Male SpCas9 CD4/CD8 Human Primary T cell P3 Nucleofection Kit TRBC1 TRBC1_site_02 75 pmol Cas9 protein; 3-fold molar excess of sgRNA

Experiment Wide Protocols

Title Description File Download SCGE ID
Tsai_T Cell Transfection Protocol Procedure for T cell transfection. Tsai_T_cell_transfection.pdf 21000000006

Associated Publications
Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI