AAVS1_site_04 | SCGE Toolkit

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SCGE ID	10000000092
Lab ID	AAVS1_site_04
Source	lab IVT
Target Locus	AAVS1
Species	human
Description	Targets AAVS1 safe harbor locus
Guide Compatibility	SpyCas9

Target Sequence	GCTCGGGGACACAGGATCCC
Target Sequence + PAM	GCTCGGGGACACAGGATCCCNGG
Position	hg38/chr19:55115851-55115874

Spacer Sequence	GCUCGGGGACACAGGAUCCC
Spacer Length	20
Modifications	none

Forward Primer	ACTGTGGGGTGGAGGGGACAGAT
Reverse Primer	AGCTCCCCTACCCCCCTTACCTCT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	1427
Guide-seq	47

Specificity Ratio	0.016

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: AAVS1_site_04 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)