B2M_site_01 | SCGE Toolkit

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SCGE ID	10000000103
Lab ID	B2M_site_01
Source	lab IVT
Target Locus	B2M
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GGCCGAGATGTCTCGCTCCG
Target Sequence + PAM	GGCCGAGATGTCTCGCTCCGNGG
Position	hg38/chr15:44711539-44711562

Spacer Sequence	GGCCGAGAUGUCUCGCUCCG
Spacer Length	20
Modifications	none

Forward Primer	CTAACCTGGCACTGCGTCGCTGG
Reverse Primer	CACAGAGGGTGCAGAGCGGGAGA

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	39
Guide-seq	1

Specificity Ratio	0.445

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: B2M_site_01 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)