SCGE ID10000000108
Lab IDB2M_site_06
Sourcelab IVT
Target LocusB2M
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGCATACTCATCTTTTTCAGT
Target Sequence + PAMGCATACTCATCTTTTTCAGTNGG
Positionhg38/chr15:44715628-44715651

Spacer SequenceGCAUACUCAUCUUUUUCAGU
Spacer Length20
Modificationsnone

Forward PrimerTGTGTCTGGGTTTCATCCATCCGACA
Reverse PrimerACTACTCATACACAACTTTCAGCAGCT


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 246
    Specificity Ratio0.079



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: B2M_site_06 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report