B2M_site_07 | SCGE Toolkit

SCGE Consortium Home | About SCGE Toolkit | Contact Us | Citing the Toolkit | License

Summary Sequence Viewer Related Editors Vector Off Targets Projects & Experiments

SCGE ID	10000000109
Lab ID	B2M_site_07
Source	lab IVT
Target Locus	B2M
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GGCATACTCATCTTTTTCAG
Target Sequence + PAM	GGCATACTCATCTTTTTCAGNGG
Position	hg38/chr15:44715629-44715652

Spacer Sequence	GGCAUACUCAUCUUUUUCAG
Spacer Length	20
Modifications	none

Forward Primer	TGTGTCTGGGTTTCATCCATCCGACA
Reverse Primer	ACTACTCATACACAACTTTCAGCAGCT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	457

Specificity Ratio	0.038

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: B2M_site_07 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)