CBLB_site_05 | SCGE Toolkit

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Summary Sequence Viewer Related Editors Vector Off Targets Projects & Experiments

SCGE ID	10000000111
Lab ID	CBLB_site_05
Source	lab IVT
Target Locus	CBLB
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GATTTCCTCCTCGACCACCA
Target Sequence + PAM	GATTTCCTCCTCGACCACCANGG
Position	hg38/chr3:105867528-105867551

Spacer Sequence	GAUUUCCUCCUCGACCACCA
Spacer Length	20
Modifications	none

Forward Primer	ATCTGCGGCAGCTTGCTTAGGGG
Reverse Primer	TGCTCTAATCGATGGAGGAAGATGCA

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	295
Guide-seq	3

Specificity Ratio	0.31

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: CBLB_site_05 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)