CBLB_site_08 | SCGE Toolkit

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SCGE ID	10000000113
Lab ID	CBLB_site_08
Source	lab IVT
Target Locus	CBLB
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GGGTATTATTGATGCTATTC
Target Sequence + PAM	GGGTATTATTGATGCTATTCNGG
Position	hg38/chr3:105867486-105867509

Spacer Sequence	GGGUAUUAUUGAUGCUAUUC
Spacer Length	20
Modifications	none

Forward Primer	AGCTTCCAAGTCTTCTCCACGGT
Reverse Primer	GGAGGAAGATGCAGTGGTACAAGT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	590

Specificity Ratio	0.017

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: CBLB_site_08 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)