CCR5_site_09 | SCGE Toolkit

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SCGE ID	10000000120
Lab ID	CCR5_site_09
Source	lab IVT
Target Locus	CCR5
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GGTACCTATCGATTGTCAGG
Target Sequence + PAM	GGTACCTATCGATTGTCAGGNGG
Position	hg38/chr3:46373261-46373284

Spacer Sequence	GGUACCUAUCGAUUGUCAGG
Spacer Length	20
Modifications	none

Forward Primer	CCTGCTCAACCTGGCCATCTCTGA
Reverse Primer	ACGCAAACACAGCCACCACCCAA

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	46
Guide-seq	6

Specificity Ratio	0.065

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: CCR5_site_09 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)