SCGE ID10000000130
Lab IDCTLA4_site_05
Sourcelab IVT
Target LocusCTLA4
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGCTTCGGCAGGCTGACAGCC
Target Sequence + PAMGCTTCGGCAGGCTGACAGCCNGG
Positionhg38/chr2:203870694-203870717

Spacer SequenceGCUUCGGCAGGCUGACAGCC
Spacer Length20
Modificationsnone

Forward PrimerCCATGCTAGCAATGCACGTGGCC
Reverse PrimerTCCCGTGTCCATGGCCCTCAGTC


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 263
    Guide-seq 1
    Specificity Ratio0.186



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: CTLA4_site_05 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report